In 3.8 M urea solution, BoNT/A LC loses most of its α-helices which convert into random coil, while the β-sheets remain intact and the tertiary structure is preserved.
In 5M urea solution, BoNT/A LC molecule regains α-helicity, yet, loses some β-sheets, and the tertiary structure changes significantly.
In 7M urea solution, BoNT/A LC unfolds completely and loses almost all its tertiary structure elements, while retaining only residual secondary structure content (α-helices).
In Confocal scan image of DR BoNT/A internalization in M17-TR2 neuroblastoma cells pre-incubated with 200 nM DR BoNT/A for 48 h.(a) Panel A. Hoechst 33342 Nucleus dye (cyan color), Panel B. Alexa fluor 594 membrane dye (red color), Panel C Alexa fluor 488 labeled DR BoNT/A (green color), Panel D. Co-localization image of all three dyes. Hoechst 33342, Alexa fluor 594, and Alexa fluor 488 was excited at excitation wavelength of 392, 594, and 488 nm respectively.
Powered by Infinite Net Solutions